Ferulic acid modulates ultraviolet-B radiation mediated inflammatory signaling in human dermal fibroblasts

Authors: Kanagalakshmi A1, Agilan B1, Mohana S1, Ananthakrishnan D2, Velmurugan D2, Karthikeyan R1, Ganesan M1, Srithar G1 and Rajendra Prasad N1*	Abstract :- (pdf) Full text :- (pdf)
Institution: 1. Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar – 608 002, India. 2. Bioinformatics Infrastructure Facility (BIF),University of Madras, Chennai-25	Google scholar Cite factor Academia.edu Issuu	Scribd Indian sciences.in Slideshare Index Copernicus

Abstract:

Ultraviolet B (UVB 290-320 nm) participate in the development of the cutaneous inflammatory response which includes a cascade of events that involves increased expression of cyclooxygenase-2 (COX-2), release of tumor necrosis factor-alpha (TNF-α) and other inflammatory cytokines. Peroxisome proliferator-activated receptors (PPARα/γ) are considered to be potential targets for photo protection because they inhibit UVB mediated inflammatory responses. In this study, we investigated the effect of ferulic acid on UVB-radiation induced expression of TNF-α and COX-2 in human dermal fibroblasts (HDFa). Further, the action of ferulic acid on PPARα/γ activation and its binding interaction with these proteins were analyzed by induced fit docking. We found that onetime UVB exposure (19.8 mJ/cm2) showed significantly increased the expressions of COX-2 and TNF-α in HDFa after 4 h post-irradiation when compared to the control cells. Ferulic acid pretreatment for 30 min before UVB exposure prevented UVB-induced overexpression of these inflammatory markers. It has also been found that ferulic acid activates PPARα/γ expressions in HDFa. Further, induced fit docking analysis showed that there was a greater binding interaction of ferulic acid with PPARγ than PPARα. Thus, ferulic acid exhibits beneficial effects against UVB-induced inflammatory responses probably through down-regulating COX-2 and TNF-α expressions and activating PPARα/γ agonists.

Key words:

Ultraviolet B radiation, Ferulic acid, Human dermal fibroblasts, Inflammatory markers, Photoprotection

How to cite: Kanagalakshmi A, Agilan B, Mohana S, Ananthakrishnan D, Velmurugan D, Karthikeyan R, Ganesan M, Srithar G and Rajendra Prasad N. Ferulic acid modulates ultraviolet-B radiation mediated inflammatory signaling in human dermal fibroblasts Journal of Research in Biology (2014) 4(8):1505-1515

Abstract: (pdf) ; Full text (pdf)

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