Detection of biofilm formation in urinary isolates: need of the hour

Authors: Saha R1*, Arora S1, Das S1, Gupta C1, Maroof KA2, Singh NP1 and Kaur IR1.	Abstract :- (.pdf) Full text :- (pdf)
Institution: 1. Department of Microbiology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Dilshad Garden, Delhi – 110095, India. 2. Department of Community Medicine, University College of Medical Sciences and Guru Teg Bahadur Hospital, Dilshad Garden, Delhi – 110095, India.	Google scholar Cite factor Academia.edu Issuu	Scribd Indian sciences.in Slideshare Index Copernicus

 Abstract:

The purpose of the study was to estimate biofilm (BF) formation in urinary catheterized patients, by comparing three methods i.e. Tissue culture plate method (TCP), Congo Red Agar method (CRM) and Tube method (TM) and to study the antimicrobial resistance pattern in BF producing and non BF producing isolates. A total of 130 urinary catheterized patients were taken as the study group. From one milli litre of urine sample isolates > 102 colony forming units per milli litre were screened for the detection of BF by TCP, TM and CRM. Antibiotic sensitivity test for both BF producing and non BF producing bacterial and fungal isolates were done as per CLSI guidelines. From 130 urine samples in our study group, 55 samples grew microorganisms of significance, of which 11 samples were poly-microbial in nature. Of these biofilm production was seen in 49 microorganisms (89.09%) by any of the three methods used. TCP method picked up 69% of biofilm producers as compared to TM and CRM which picked up only 36% and 27% biofilm producers respectively. Our study reveals TCP method as the more dependable one as compared to TM and CRA methods for the quantitative biofilm detection, so it can be recommended as a screening method in laboratories.

Key words:

Biofilm, biofilm detection, Congo Red Agar.

Abbreviations:

BF - Biofilms; TCP - Tissue Culture Plate; CRM - Congo Red Method; TM - Tube Method; CLSI - Clinical Laboratory Standard Institute; CAUTI - Catheter associated Urinary Tract Infection; CLED - Cysteine Lactose Electrolyte Deficient; BHIB - Brain Heart Infusion Broth; TSB - Trypticase soy broth; ELISA - Enzyme linked immunosorbent assay; MHA - Muller Hinton Agar; MIC -Minimum Inhibitory Concentration; ATCC - American type culture collection; GPC -Gram positive cocci; GNB - Gram negative bacilli.

How to cite: Saha R, Arora S, Das S, Gupta C, Maroof KA, Singh NP and Kaur IR. Detection of biofilm formation in urinary isolates: need of the hour. Journal of Research in Biology (2014) 4(1): 1174-1181

Abstract: (pdf) ; Full text (pdf)

License Agreement:

This article is governed by the Creative Commons Attribution License (http://creativecommons.org/ licenses/by/4.0), which gives permission for unrestricted use, non-commercial, distribution and reproduction in all medium, provided the original work is properly cited